Nucleotide Sequence of the Gene Encoding β-Isopropylmalate Dehydrogenase (leuB) from Bacteroides fragilis

Abstract

The complete nucleotide sequence of the gene (leuB) coding for β-isopropylmalate dehydrogenase of Bacteroides fragilis was determined. An open reading frame of 1, 061 nucleotides was detected that could encode a polypeptide of 353 amino acid residues with a calculated molecular mass of 39, 179 Da. The deduced amino acid sequence of the β-isopropylmalate dehydrogenase from B. fragilis showed substantial sequence similarity with the β-isopropylmalate dehydrogenases from other bacteria.