Detection of Treponema socranskii Associated with Human Periodontitis by PCR

Mitsuo Sakamoto; Yasuo Takeuchi; Makoto Umeda; Isao Ishikawa; Yoshimi Benno; Takashi Nakase

Detection of Treponema socranskii Associated with Human Periodontitis by PCR

Mitsuo Sakamoto, Yasuo Takeuchi, Makoto Umeda, Isao Ishikawa, Yoshimi Benno, Takashi Nakase

Author information

Keywords: Treponema socranskii, Periodontitis, PCR

JOURNAL FREE ACCESS

1999 Volume 43 Issue 5 Pages 485-490

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Published: May 20, 1999 Received: December 18, 1998 Available on J-STAGE: March 17, 2008 Accepted: February 19, 1999 Advance online publication: - Revised: -
Correction information
Date of correction: March 17, 2008 Reason for correction: - Correction: TITLE Details: Wrong : Detection of Treponema socranskii Associated with Human Periodontitis by PCR
Right : Detection of Treponema socranskii Associated with Human Periodontitis by PCR

Date of correction: March 17, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : A PCR technique was used to detect and identify Treponema socranskii associated with periodontitis. A species-specific forward primer was designed for a variable region within its 16S rRNA gene and was used in conjunction with a conserved reverse primer. This primer pair was tested for specificity against 44 oral bacterial strains. Sensitivity was determined using a serial dilution of T. socranskii cells. Amplification products were obtained from all T. socranskii strains tested, but not from other oral bacteria associated with periodontal disease. The detection limit of PCR was 5 T. socranskii cells per PCR. T. socranskii was detected by PCR in subgingival plaque and saliva samples from patients with periodontitis.
Right : A PCR technique was used to detect and identify Treponema socranskii associated with periodontitis. A species-specific forward primer was designed for a variable region within its 16S rRNA gene and was used in conjunction with a conserved reverse primer. This primer pair was tested for specificity against 44 oral bacterial strains. Sensitivity was determined using a serial dilution of T. socranskii cells. Amplification products were obtained from all T. socranskii strains tested, but not from other oral bacteria associated with periodontal disease. The detection limit of PCR was 5 T. socranskii cells per PCR. T. socranskii was detected by PCR in subgingival plaque and saliva samples from patients with periodontitis.

Date of correction: March 17, 2008 Reason for correction: - Correction: DTPUB Details: Right : 19990520

Date of correction: March 17, 2008 Reason for correction: - Correction: AFFILIATION Details: Wrong :
1) Japan Collection of Microorganisms, The Institute of Physical and Chemical Research
2) Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University

Right :
1) Japan Collection of Microorganisms, The Institute of Physical and Chemical Research (RIKEN)
2) Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University

Date of correction: March 17, 2008 Reason for correction: - Correction: KEYWORD Details: Wrong : Treponema socranskii, Periodontitis, PCR
Right : Treponema socranskii, Periodontitis, PCR

Date of correction: March 17, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 1) Benkirane, R.M., Guillot, E., and Mouton, C. 1995. Immunomagnetic PCR and DNA probe for detection and identification of Porphyromonas gingivalis. J. Clin. Microbiol. 33: 2908-2912.
2) Chan, E.C.S., Siboo, R., Keng, T., Psarra, N., Hurley, R., Cheng, S.L., and Iugovaz, I. 1993. Treponema denticola (ex Brumpt 1925) sp. nov., nom. rev., and identification of new spirochete isolates from periodontal pockets. Int. J. Syst. Bacteriol. 43: 196-203.
3) Choi, B.K., Paster, B.J., Dewhirst, F.E., and Göbel, U.B. 1994. Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis. Infect. Immun. 62: 1889-1895.
4) Dangtuan, S., and Rudney, J.D. 1996. Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J. Clin. Microbiol. 34: 2674-2678.
5) DiRienzo, J.M., Cornell, S., and Boehringer, H. 1991. Use of randomly cloned DNA fragments for the identification of oral spirochetes. Oral Microbiol. Immunol. 6: 88-96.
6) Fremming, T.F., Rüdiger, S., Hofmann, U., Schmidt, H., Plaschke, B., Strätz, A., Klaiber, B., and Karch, H. 1995. Identification of Actinobacillus actinomycetemcomitans in subgingival plaque by PCR. J. Clin. Microbiol. 33: 3102-3105.
7) Koseki, T., Ishikawa, I., Umeda, M., and Benno, Y. 1995. Characterization of oral treponemes isolated from human periodontal pockets. Oral Microbiol. Immunol. 10: 271-277.
8) Maidak, B.L., Olsen, G.J., Larsen, N., Overbeek, R., McCaughey, M.J., and Woese, C.R. 1997. The RDP (Ribosomal Database Project). Nucleic Acids Res. 25: 109-111.
9) Mättö, J., Saarela, M., Alaluusua, S., Oja, V., Jousimies-Somer, H., and Asikainen, S. 1998. Detection of Porphyromonas gingivalis from saliva by PCR by using a simple sample-processing method. J. Clin. Microbiol. 36: 157-160.
10) McClellan, D.L., Griffen, A.L., and Leys, E.J. 1996. Age and prevalence of Porphyromonas gingivalis in children. J. Clin. Microbiol. 34: 2017-2019.
11) Moore, W.E.C., Holdeman, L.V., Smibert, R.M., Cato, E.P., Burmeister, J.A., Palcanis, K.G., and Ranney, R.R. 1984. Bacteriology of experimental gingivitis in children. Infect. Immun. 46: 1-6.
12) Moore, W.E.C., Holdeman, L.V., Smibert, R.M., Hash, D.E., Burmeister, J.A., and Ranney, R.R. 1982. Bacteriology of severe periodontitis in young adult humans. Infect. Immun. 38: 1137-1148.
13) Moter, A., Hoenig, C., Choi, B.K., Riep, B., and Göbel, U.B. 1998. Molecular epidemiology of oral treponemes associated with periodontal disease. J. Clin. Microbiol. 36: 1399-1403.
14) Riviere, G.R., and DeRouen, T.A. 1998. Association of oral spirochetes from periodontally healthy sites with development of gingivitis. J. Periodontol. 69: 496-501.
15) Riviere, G.R., DeRouen, T.A., Kay, S.L., Avera, S.P., Stouffer, V.K., and Hawkins, N.R. 1997. Association of oral spirochetes from sites of periodontal health with development of periodontitis. J. Periodontol. 68: 1210-1214.
16) Riviere, G.R., Smith, K.S., Carranza, N., Jr., Tzagaroulaki, E., Kay, S.L., and Dock, M. 1995. Subgingival distribution of Treponema denticola, Treponenia socranskii, and pathogenrelated oral spirochetes: prevalence and relationship to periodontal status of sampled sites. J. Periodontol. 66: 829-837.
17) Riviere, G.R., Thompson, A.J., Brannan, R.D., McCoy, D.E., and Simonson, L.G. 1996. Detection of pathogenrelated oral spirochetes, Treponema denticola, and Treponema socranskii in dental plaque from dogs. J. Vet. Dent. 13: 135-138.
18) Smibert, R.M., and Burmeister, J.A. 1983. Treponema pectinovorum sp. nov. isolated from humans with periodontitis. Int. J. Syst. Bacteriol. 33: 852-856.
19) Smibert, R.M., Johnson, J.L., and Ranney, R.R. 1984. Treponema socranskii sp. nov., Treponema socranskii subsp. socranskii subsp. nov., Treponema socranskii subsp. buccale subsp. nov., and Treponema socranskii subsp. paredis subsp. nov. isolated from the human periodontia. Int. J. Syst. Bacteriol. 34: 457-462.
20) Thompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.
21) Umemoto, T., Li, M., and Namikawa, I. 1997. Adherence of human oral spirochetes by collagen-binding proteins. Microbiol. Immunol. 41: 917-923.
22) Umemoto, T., Nakazawa, F., Hoshino, E., Okada, K., Fukunaga, M., and Namikawa, I. 1997. Treponema medium sp. nov., isolated from human subgingival dental plaque. Int. J. Syst. Bacteriol. 47: 67-72.
23) Umemoto, T., and Namikawa, I. 1994. Binding of hostassociated treponeme proteins to collagens and laminin: a possible mechanism of spirochetal adherence to host tissues. Microbiol. Immunol. 38: 655-663.
24) Wyss, C., Choi, B.K., Schüpbach, P., Guggenheim, B., and Göbel, U.B. 1996. Treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions. Int. J. Syst. Bacteriol. 46: 745-752.
25) Wyss, C., Choi, B.K., Schüpbach, P., Guggenheim, B., and Göbel, U.B. 1997. Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int. J. Syst. Bacteriol. 47: 842-845.

Right : 1) Benkirane, R.M., Guillot, E., and Mouton, C. 1995. Immunomagnetic PCR and DNA probe for detection and identification of Porphyromonas gingivalis. J. Clin. Microbiol. 33 : 2908-2912.
2) Chan, E.C.S., Siboo, R., Keng, T., Psarra, N., Hurley, R., Cheng, S.L., and Iugovaz, I. 1993. Treponema denticola (ex Brumpt 1925) sp. nov., nom. rev., and identification of new spirochete isolates from periodontal pockets. Int. J. Syst. Bacteriol. 43 : 196-203.
3) Choi, B.K., Paster, B.J., Dewhirst, F.E., and Göel, U.B.1994. Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis. Infect. Immun. 62 : 1889-1895.
4) Dangtuan, S., and Rudney, J.D. 1996. Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J. Clin. Microbiol. 34 : 2674-2678.
5) DiRienzo, J.M., Cornell, S., and Boehringer, H. 1991. Use of randomly cloned DNA fragments for the identification of oral spirochetes. Oral Microbiol. Immunol. 6 : 88-96.
6) Fremming, T.F., Rudiger, S., Hofmann, U., Schmidt, H., Plaschke, B., Stratz, A., Klaiber, B., and Karch, H. 1995. Identification of Actinobacillus actinomycetemcomitans in subgingival plaque by PCR. J. Clin. Microbiol. 33 : 3102-3105.
7) Koseki, T., Ishikawa, I., Umeda, M., and Benno, Y. 1995. Characterization of oral treponemes isolated from human periodontal pockets. Oral Microbiol. Immunol. 10 : 271-277.
8) Maidak, B.L., Olsen, G.J., Larsen, N., Overbeek, R., McCaughey, M.J., and Woese, C.R. 1997. The RDP (Ribosomal Database Project). Nucleic Acids Res. 25 : 109-111.
9) Matto, J., Saarela, M., Alaluusua, S., Oja, V., JousimiesSomer, H., and Asikainen, S. 1998. Detection of Porphyromonas gingivalis from saliva by PCR by using a simple sample-processing method. J. Clin. Microbiol. 36 : 157-160.
10) McClellan, D.L., Griffen, A.L., and Leys, E.J. 1996. Age and prevalence of Porphyromonas gingivalis in children. J. Clin. Microbiol. 34 : 2017-2019.
11) Moore, W.E.C., Holdeman, L.V., Smibert, R.M., Cato, E.P., Burmeister, J.A., Palcanis, K.G., and Ranney, R.R. 1984. Bacteriology of experimental gingivitis in children. Infect. Immun. 46 : 1-6.
12) Moore, W.E.C., Holdeman, L.V., Smibert, R.M., Hash, D.E., Burmeister, J.A., and Ranney, R.R. 1982. Bacteriology of severe periodontitis in young adult humans. Infect. Immun. 38 : 1137-1148.
13) Moter, A., Hoenig, C., Choi, B.K., Riep, B., and Gobel, U.B. 1998. Molecular epidemiology of oral treponemes associated with periodontal disease. J. Clin. Microbiol. 36 : 1399-1403.
14) Riviere, G.R., and DeRouen, T.A. 1998. Association of oral spirochetes from periodontally healthy sites with development of gingivitis. J. Periodontol. 69 : 496-501.
15) Riviere, G.R., DeRouen, T.A., Kay, S.L., Avera, S.P., Stouffer, V.K., and Hawkins, N.R. 1997. Association of oral spirochetes from sites of periodontal health with development of periodontitis. J. Periodontol. 68 : 1210-1214.
16) Riviere, G.R., Smith, K.S., Carranza, N., Jr., Tzagaroulaki, E., Kay, S.L., and Dock, M. 1995. Subgingival distribution of Treponema denticola, Treponema socranskii, and pathogenrelated oral spirochetes : prevalence and relationship to periodontal status of sampled sites. J. Periodontol. 66 : 829-837.
17) Riviere, G.R., Thompson, A.J., Brannan, R.D., McCoy, D.E., and Simonson, L.G. 1996. Detection of pathogenrelated oral spirochetes, Treponema denticola, and Treponema socranskii in dental plaque from dogs. J. Vet. Dent.13 : 135-138.
18) Smibert, R.M., and Burmeister, J.A. 1983. Treponema pectinovorum sp. nov. isolated from humans with periodontitis. Int. J. Syst. Bacteriol. 33 : 852-856.
19) Smibert, R.M., Johnson, J.L., and Ranney, R.R. 1984. Treponema socranskii sp. nov., Treponema socranskii subsp. socranskii subsp. nov., Treponema socranskii subsp. buccale subsp. nov., and Treponema socranskii subsp. paredis subsp. nov. isolated from the human periodontia. Int. J. Syst. Bacteriol. 34 : 457-462.
20) Thompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTAL W : improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22 : 4673-4680.
21) Umemoto, T., Li, M., and Namikawa, I. 1997. Adherence of human oral spirochetes by collagen-binding proteins. Microbiol. Immunol. 41 : 917-923.
22) Umemoto, T., Nakazawa, F., Hoshino, E., Okada, K., Fukunaga, M., and Namikawa, I. 1997. Treponema medium sp. nov., isolated from human subgingival dental plaque. Int. J. Syst. Bacteriol. 47 : 67-72.
23) Umemoto, T., and Namikawa, I. 1994. Binding of hostassociated treponeme proteins to collagens and laminin : a possible mechanism of spirochetal adherence to host tissues. Microbiol. Immunol. 38 : 655-663.
24) Wyss, C., Choi, B.K., SchUpbach, P., Guggenheim, B., and Gobel, U.B. 1996. Treponema maltophilum sp. nov., a small oral spirochete isolated from human periodontal lesions. Int. J. Syst. Bacteriol. 46 : 745-752.
25) Wyss, C., Choi, B.K., Schüpbach, P., Guggenheim, B., and Göbel, U.B. 1997. Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int. J. Syst. Bacteriol. 47 : 842-845.

Abstract

A PCR technique was used to detect and identify Treponema socranskii associated with periodontitis. A species-specific forward primer was designed for a variable region within its 16S rRNA gene and was used in conjunction with a conserved reverse primer. This primer pair was tested for specificity against 44 oral bacterial strains. Sensitivity was determined using a serial dilution of T. socranskii cells. Amplification products were obtained from all T. socranskii strains tested, but not from other oral bacteria associated with periodontal disease. The detection limit of PCR was 5 T. socranskii cells per PCR. T. socranskii was detected by PCR in subgingival plaque and saliva samples from patients with periodontitis.

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