Genotyping of Pneumocystis carinii f. sp. hominis Isolates in Japan Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes

Noriaki Hosoya; Takashi Takahashi; Miki Wada; Tokiomi Endo; Tetsuya Nakamura; Hiroyuki Sakashita; Kyoko Kimura; Kenji Ohnishi; Yoshikazu Nakamura; Tsuguo Mizuochi; Aikichi Iwamoto

Genotyping of Pneumocystis carinii f. sp. hominis Isolates in Japan Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes

Noriaki Hosoya, Takashi Takahashi, Miki Wada, Tokiomi Endo, Tetsuya Nakamura, Hiroyuki Sakashita, Kyoko Kimura, Kenji Ohnishi, Yoshikazu Nakamura, Tsuguo Mizuochi, Aikichi Iwamoto

Author information

Noriaki Hosoya
Department of Infectious Diseases, Institute of Medical Science, The University of Tokyo
Laboratory of Biomedical Chemistry, Department of Applied Chemistry, Tokai University
Takashi Takahashi
Department of Infectious Diseases, Institute of Medical Science, The University of Tokyo
Miki Wada
Department of Tumor Biology, Institute of Medical Science, The University of Tokyo
Tokiomi Endo
Department of Infectious Diseases, Institute of Medical Science, The University of Tokyo
Tetsuya Nakamura
Department of Infectious Diseases and Applied Immunology, Institute of Medical Science, The University of Tokyo
Hiroyuki Sakashita
Department of Internal Medicine, Tokyo Metropolitan Bokutoh General Hospital
Kyoko Kimura
Department of Infectious Diseases, Tokyo Metropolitan Bokutoh General Hospital
Kenji Ohnishi
Department of Infectious Diseases, Tokyo Metropolitan Bokutoh General Hospital
Yoshikazu Nakamura
Department of Tumor Biology, Institute of Medical Science, The University of Tokyo
Tsuguo Mizuochi
Laboratory of Biomedical Chemistry, Department of Applied Chemistry, Tokai University
Aikichi Iwamoto
Department of Infectious Diseases, Institute of Medical Science, The University of Tokyo
Department of Infectious Diseases and Applied Immunology, Institute of Medical Science, The University of Tokyo

Keywords: Pneumocystis carinii (Pc), Genotyping, Internal transcribed spacer (ITS), rRNA

JOURNAL FREE ACCESS

2000 Volume 44 Issue 7 Pages 591-596

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Abstract

Genotyping of Pneumocystis carinii (Pc) isolated from 24 bronchoalveolar lavage (BAL) fluid specimens in Japan was examined based on nucleotide sequence variations in internal transcribed spacer regions 1 and 2 (ITS1 and ITS2, respectively) of rRNA genes. We found 11 ITS1 genotypes including 2 novel ones and 11 ITS2 genotypes including 3 new ones. Combining the ITS1 and ITS2 genotypes resulted in 30 ITS genotypes, of which 10 are newly described in this report. Two or more genotypes in ITS regions in a specimen were observed in 16 of 24 patients. Our results will be of help for the epidemiological investigation of Pc infection.

Corresponding author

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