Adherence Protects the Binding Sites of Helicobacter pylori Urease from Acid-Induced Damage

Faustino C. Icatlo Jr; Hideaki Yokoyama; Masahiko Kuroki; Chizu Kobayashi; Hideo Goshima; Yutaka Ikemori; Yoshikatsu Kodama

Adherence Protects the Binding Sites of Helicobacter pylori Urease from Acid-Induced Damage

Faustino C. Icatlo Jr, Hideaki Yokoyama, Masahiko Kuroki, Chizu Kobayashi, Hideo Goshima, Yutaka Ikemori, Yoshikatsu Kodama

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Keywords: Helicobacter pylori, Urease, Adherence, Acid

JOURNAL FREE ACCESS

2000 Volume 44 Issue 9 Pages 773-776

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Published: September 20, 2000 Received: March 13, 2000 Available on J-STAGE: March 17, 2008 Accepted: June 20, 2000 Advance online publication: - Revised: -
Correction information
Date of correction: March 17, 2008 Reason for correction: - Correction: TITLE Details: Wrong : Adherence Protects the Binding Sites of Helicobacter pylori Urease from Acid-Induced Damage
Right : Adherence Protects the Binding Sites of Helicobacter pylori Urease from Acid-Induced Damage

Date of correction: March 17, 2008 Reason for correction: - Correction: ABSTRACT Details: Wrong : Colonization by Helicobacter pylori partly depends on acid-dependent adherence by urease to gastric mucin. To further verify the relevance of urease adherence to colonization, the influence of acidity on the binding sites of H. pylori urease was investigated. When enzyme-based in vitro ligand capture assays were used, the effect of acidity on the binding site of H. pylori urease was determined against a backdrop medium consisting of acidic buffers simulating the luminal side of gastric mucus. A high degree of stability was exhibited by adherent urease, suggesting a pivotal role by the denatured enzyme in the persistence of the bacterium within the acidified compartment of gastric mucus.
Right : Colonization by Helicobacter pylori partly depends on acid-dependent adherence by urease to gastric mucin. To further verify the relevance of urease adherence to colonization, the influence of acidity on the binding sites of H. pylori urease was investigated. When enzyme-based in vitro ligand capture assays were used, the effect of acidity on the binding site of H. pylori urease was determined against a backdrop medium consisting of acidic buffers simulating the luminal side of gastric mucus. A high degree of stability was exhibited by adherent urease, suggesting a pivotal role by the denatured enzyme in the persistence of the bacterium within the acidified compartment of gastric mucus.

Date of correction: March 17, 2008 Reason for correction: - Correction: DTPUB Details: Right : 20000920

Date of correction: March 17, 2008 Reason for correction: - Correction: AUTHOR Details: Wrong : Faustino C. Icatlo Jr.¹⁾, Hideaki Yokoyama¹⁾, Masahiko Kuroki¹⁾, Chizu Kobayashi¹⁾, Hideo Goshima¹⁾, Yutaka Ikemori¹⁾, Yoshikatsu Kodama¹⁾
Right : Faustino C. Icatlo Jr¹⁾, Hideaki Yokoyama¹⁾, Masahiko Kuroki¹⁾, Chizu Kobayashi¹⁾, Hideo Goshima¹⁾, Yutaka Ikemori¹⁾, Yoshikatsu Kodama¹⁾

Date of correction: March 17, 2008 Reason for correction: - Correction: KEYWORD Details: Wrong : Helicobacter pylori, Urease, Adherence, Acid
Right : Helicobacter pylori, Urease, Adherence, Acid

Date of correction: March 17, 2008 Reason for correction: - Correction: CITATION Details: Wrong : 1) Axon, A.T. 1993. Helicobacter pylori infection. J. Antimicrob. Chemother. 32 (Suppl. A) 61-68.
2) Bauerfind, P., Garner, R., Dunn, B.E., and Mobley, H.L. 1997. Synthesis and activity of Helicobacter pylori urease and catalase at low pH. Gut 440: 25-30.
3) Bijlsma, J.J.E., Gerrits, M.M., Imandi, R., Vandenbroucke-Grants, C.M.J.E., and Kusters, J.G. 1998. Urease-positive, acid-sensitive mutants of Helicobacter pylori: urease-independent acid-resistance involved in growth at low pH. FEMS Microbiol. Lett. 167: 309-313.
4) Icatlo, F.C., Jr., Kuroki, M., Kobayashi, C., Yokoyama, H., Ikemori, Y., Hashi, T., and Kodama, Y. 1998. Affinity purification of Helicobacter pylori urease: relevance to gastric mucin adherence by urease protein. J. Biol. Chem. 273: 18130-18138.
5) Icatlo, F.C., Jr., Goshima, H., Kimura, N., and Kodama, Y. 2000. Acid-dependent adherence of Helicobacter pylori urease to diverse polysaccharides. Gastroenterology (in press).
6) Marshall, B.J. 1993. Helicobacter pylori: a primer for 1994. Gastroenterologist 1: 241-247.
7) Matin, A., Zychlinsky, E., Keyhan, M., and Sachs, G. 1996.Capacity of Helicobacter pylori to generate ionic gradients at low pH is similar to that of bacteria which grow under strongly acidic conditions. Infect. Immun. 64: 1434-1436.
8) McGowan, C.C., Cover, T.L., and Blaser, M.J. 1994. The proton pump inhibitor omeprazole inhibits acid survival of Helicobacter pylori by a urease-independent mechanism. Gastroenterology 107: 1573-1578.
9) Rektorschek, M., Weeks, D., Sachs, G., and Melchers, K. 1998. Influence of pH on metabolism and urease activity of Helicobacter pylori. Gastroenterology 115: 628-641.
10) Scott, D.R., Weeks, D., Hong, C., Postius, S., Melchers, K., and Sachs, G. 1998. The role of internal urease in acid resistance of Helicobacter pylori. Gastroenterology 114: 58-70.

Right : 1) Axon, A.T. 1993. Helicobacter pylori infection. J. Antimicrob. Chemother. 32 (Suppl. A) 61-68.
2) Bauerfind, P., Garner, R., Dunn, B.E., and Mobley, H.L. 1997. Synthesis and activity of Helicobacter pylori urease and catalase at low pH. Gut 440 : 25-30.
3) Bijlsma, J.J.E., Gerrits, M.M., Imandi, R., VandenbrouckeGrants, C.M.J.E., and Kusters, J.G. 1998. Urease-positive, acid-sensitive mutants of Helicobacter pylori : urease-independent acid-resistance involved in growth at low pH. FEMS Microbiol. Lett. 167 : 309-313.
4) Icatlo, F.C., Jr., Kuroki, M., Kobayashi, C., Yokoyama, H., Ikemori, Y., Hashi, T., and Kodama, Y. 1998. Affinity purification of Helicobacter pylori urease : relevance to gastric mucin adherence by urease protein. J. Biol. Chem. 273 : 18130-18138.
5) Icatlo, F.C., Jr., Goshima, H., Kimura, N., and Kodama, Y. 2000. Acid-depexendent adherence, of Helicobacter pylori urease to diverse polysaccharides. Gastroenterology (in press).
6) Marshall, B.J. 1993. Helicobacter pylori : a primer for 1994. Gastroenterologist 1 : 241-247.
7) Matin, A., Zychlinsky, E., Keyhan, M., and Sachs, G. 1996. Capacity of Helicobacter pylori to generate ionic gradients at low pH is similar to that of bacteria which grow under strongly acidic conditions. Infect. Immun. 64 : 1434-1436.
8) McGowan, C.C., Cover, T.L., and Blaser, M.J. 1994. The proton pump inhibitor omeprazole inhibits acid survival of Helicobacter pylori by a urease-independent mechanism. Gastroenterology 107 : 1573-1578.
9) Rektorschek, M., Weeks, D., Sachs, G., and Melchers, K. 1998. Influence of pH on metabolism and urease activity of Helicobacter pylori. Gastroenterology 115 : 628-641.
10) Scott, D.R., Weeks, D., Hong, C., Postius, S., Melchers, K., and Sachs, G. 1998. The role of internal urease in acid resistance of Helicobacter pylori. Gastroenterology 114 : 58-70.

Abstract

Colonization by Helicobacter pylori partly depends on acid-dependent adherence by urease to gastric mucin. To further verify the relevance of urease adherence to colonization, the influence of acidity on the binding sites of H. pylori urease was investigated. When enzyme-based in vitro ligand capture assays were used, the effect of acidity on the binding site of H. pylori urease was determined against a backdrop medium consisting of acidic buffers simulating the luminal side of gastric mucus. A high degree of stability was exhibited by adherent urease, suggesting a pivotal role by the denatured enzyme in the persistence of the bacterium within the acidified compartment of gastric mucus.

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