Abstract
Current antigen retrieval techniques include the use of citrate buffer, Tris-HCl containing 5% urea, and EDTA solutions combined with heating in a microwave oven or autoclave. These methods must be adjusted for a given tissue or antigen. To improve the efficiency of antigen retrieval for immunohistochemical staining, we developed a new method using citraconic anhydride. We describe this new antigen retrieval method using 0.05% citraconic anhydride solution of pH 7.4 and heat. This antigen retrieval method produced satisfactory staining results for a wide variety of antigens.
