Nihon Ika Daigaku Igakkai Zasshi
Online ISSN : 1880-2877
Print ISSN : 1349-8975
ISSN-L : 1349-8975
Lessons from Basic Research
3. Gene Delivery and Expression Series Gene Delivery and Expression (1)
Hideo Orimo
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2011 Volume 7 Issue 2 Pages 92-96

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Abstract
The delivery of genes into cells is a basic procedure in molecular biology. The induction of genes into prokaryotic cells is referred to as transformation, which is a basic method in gene cloning, whereas that into eukaryotic cells is called transfection. Several transfection methods have been reported: physical, chemical, and biological. In this technical note, a transfection method using cationic lipids is described for studies of gene function and the expression of mutant proteins.
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© 2011 by the Medical Association of Nippon Medical School
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