Molecular Secondary Ion Mass Spectrometry of Oligosaccharides Assisted by Amide Matrices

Abstract

In order to seek for a good matrix material for neutral oligosaccharides instead of DEA in molecular SIMS, we examined seven amide compounds which possess intermediate proton affinity between glycerol and DEA. Among them, HEF, PR and PP were available for our purposes. The amide matrices-assisted mass spectra give surely both molecular adduct ions ([M + × + H]⁺, × = HEF, PR and PP) and some sequence ions. Additionally, the metastable ion spectra of [M + × + H]⁺ under linked scanning (B/E) conditions gave structurally reliable information.