Abstract
Liquid chromatographic influence on changing the solvent system from the non-volatile buffer containing inorganic salts or phosphates to some volatile buffers suitable for the direct coupled liquid chromatography/mass spectrometry was investigated by means of the reversed-phase high-performance liquid chromatography. On a separation of ten methylxanthines including methyluric acids, almost the same chromatograms of the mixture were obtained among all buffers examined. Similar modifications on the solvent system were tried to separate a mixture of nine non-steroidal anti-inflammatory drugs and anthranilic acid. In an application of the modified buffer to the FRIT-FAB liquid chromatography/mass spectrometry, informative negative ion mass spectra of acetyl-coenzyme A and structurally related compounds could be obtained by using diethanolamine-acetic acid buffer. Diethanolamine, its acetate in the buffer, acts effectively as a matrix for the FRIT-FAB liquid chromatography/mass spectrometry.
