Abstract
Antigenic determinants, also called epitopes, constitute the region of the protein in intimate contact with the antigen-binding region of an antibody. Epitopes are typically classified as being either continuous or discontinuous. A variety of methods have been applied to the study of monoclonal antibody (mAb)-antigen interactions and the characterization of their respective epitopes. Here we introduce some new methods of peptide and protein epitope mapping by mass spectrometry.
