Structural Analysis of Two Pyoverdins by Electrospray and FAB Mass Spectrometry

Abstract

Pyoverdins are chromopeptides produced by the so-called fluorescent group of the bacterial genus Pseudomonas. Species or even strain specific they differ in the composition of their peptide chains. For classification purposes it is of importance to get fast information regarding the nature of the latter. For two new representatives from hydrolysis results and especially from an analysis of the data obtained from electrospray ionization (ESI) and fast atom bombardement (FAB) mass spectrometry measurements the amino acid sequences can be suggested as Asp-BuOHOrn-Dab-aThr-Gly-Ser-Ser-OHAsp-aThr (Pseudomonas putida BTP16) and Ser-Ser-FoOHOrn-Ser-Ser-Ser-FoOHOrn-Lys-Lys (Pseudomonas fluorescens BTP7).