Abstract
The quantitative analysis is the base of clinical pharmacokinetics, pharmacokinetics, toxicokinetics, and pharmacodynamics. The pretreatment is important for the quantitative analysis, because the recovery of the pretreatment for the quantitative analysis affects the biological concentration of the drug. The pretreatment has many functions such as the change of time, place, and men, etc. from sampling to the analysis. The stability and adsorption of the drug during the pretreatment affects the quantitative values. The biological matrix such as plasma, urine, bile, feces, and tissues interferes the analysis. The organic solvents and water is purified before using for the pretreatment. The plastic containers for sampling and stocking, the extraction tube and HPLC, tube contains the interfere peaks such as dioctylphthalate. The technique of the pretreatment such as the liquidliquid extraction, solid phase extraction, open column extraction, TLC, HPLC, and LC/MS are shown. The maintenance of LC/MS affects the quantitative values. The problems and the countermeasure of the pretreatment for the quantitative analysis are described.
