Abstract
Determination of low molecular weight compounds by MALDI-TOF MS was developed using emedastine, an anti-allergic drug, as model compound. It was found that α-cyano-4-hydroxycinnamic acid is suitable as matrix and a mixture of acetonitrile and water (7:3) as solvent for the measurement. Emedastine in rat plasma was extracted with mixture of hexane and chloroform (7:3). Calibration curve of emedastine in rat plasma was linear over the range 5-100 ng/mL with coefficient of 0.997. It seems that MALDI-TOF MS is useful for the determination of a drug in plasma.
