Abstract
The “in-gel” digestion followed by peptide mass fingerprint is an innovative method of identifying the proteins separated by one- or two-dimensional polyacrylamide gel electrophoresis. It is now widely used in various applications in proteomics, e.g., differential display of proteins in specific biological conditions, or exhaustive identification of the components that physically interact in a protein complex. In this NOTE, the standard procedures including silver-staining and in-gel digestion are described, and the peptides derived from skin keratins that are often mixed in the process are listed.
