Mechanism for the Degardation of Ribostamycin by the Laser Spray

Abstract

A laser spray interface for use in liquid chromatography/mass spectrometry (LC/MS) has been investigated with respect to the degradation of a thermally labile compound, ribostamycin. It was confirmed that few fragment ions were formed when the laser beam was focused to the center of the stainless steel capillary. When the laser beam was slightly off-centered, the sample ions suffered from the thermal degradation by the heated wall of the stainless steel capillary. A feasible observation of fragment ions for the thermally labile compounds by the wall heating would be useful for the structural elucidation of the sample molecules.