Evaluation of Solution Phase Isoelectrofocusing as Part of Proteomics Strategies

Abstract

Solution isoelectrofocusing has been evaluated for prefractionation of nuclear proteins from human cancer MCF-7 cells. Two separation devices were exercised using commercial polyacrylamide membranes, which carry embedded immobiline mixtures to establish pH boundaries between chambers. Protein recovery was quantified and 2-D gel electrophoresis was used to evaluate resolution. Solution isoelectrofocusing provides satisfactory separations and decreases the complexity of the protein mixture, however rates of recovery need to be improved.