Abstract
We have developed a high-throughput strategy for focused absolute quantitative proteomics using multi-channel selected-reaction-monitoring mass-spectrometric analysis with in silico peptide selection criteria, and confirmed its quantitative accuracy and reproducibility. The method was applied to examine simultaneously the expression levels of 34 drug transporter proteins, which have been reported to be functionally expressed in the relevant tissues, in mouse tissues. The developed method will provide an inclusive assay platform for all transporter proteins expressed both in animal/human tissues and would contribute progress of drug discovery and development.
