Material Cycles and Waste Management Research
Online ISSN : 2187-4808
Print ISSN : 1883-5864
ISSN-L : 1883-5864
Special Issue: Asbestos Issues: Present and Future
Development of Fluorescence Microscopy Method to Detect Airborne Asbestos and the Challenge of Automation
Akio Kuroda Takenori IshidaTomoki Nishimura
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2020 Volume 31 Issue 5 Pages 345-351

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Abstract
The current method for detecting asbestos fibers in air relies on a combination of phase-contrast and electron microscopy. Since this analysis is time-consuming and requires highly skilled operators, as well as bulky equipment, it is not suitable for on-site asbestos testing. The authors have developed the fluorescence microscopy (FM) method, which relies on fluorescence probes that can selectively bind to asbestos. A high correlation was observed between test results presented by the FM method and those of the electron microscopy-based method. In 2017, Japan’s Ministry of the Environment approved the FM method as a rapid analytical method for asbestos release at demolition sites. To address the demand for on-site detection of asbestos, we developed a portable fluorescent microscope that is robust enough to tolerate outdoor use. The FM method using an air sampling/fluorescent staining equipment and the portable fluorescent microscope can provide results within an hour at demolition sites. The authors are currently using an AI image analysis in order to develop a fully automatic airborne asbestos detection system.
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© 2020 Japan Society of Material Cycles and Waste Management
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