Abstract
Anticoagulant activities of the salivary glands (SG) of the blood sucking kissing bug, Rhodnius prolixus, were analyzed. The crude SG extract showed strong activity to prolong activated partial thromboplastin time (APTT) and also weak activity in elongation of prothrombin time (PT). Inhibition activities of SG extract to the factors in the intrinsic and common coagulation pathways were analyzed. SG extract showed high inhibition activity to the IXa/VIIIa/phospholipid (PL) complex (Xase complex) and low to serine protease, kallikrein, factor XIIa, and factor IIa. However SG extract did not show inhibition activity to factors XIa and Xa. These results indicate that SG contains a strong inhibitor of Xase complex in the intrinsic coagulation pathway and an inhibitor of factor IIa in the common pathway. Both anticoagulant factors were separated into two fractions by gel filtration (GF).
