Medical Entomology and Zoology
Online ISSN : 2185-5609
Print ISSN : 0424-7086
Original Articles
Construction of a DNA database for ticks collected in Japan: application of molecular identification based on the mitochondrial 16S rDNA gene
Ai TAKANOHiromi FUJITATeruki KADOSAKAMamoru TAKAHASHITakeo YAMAUCHIFubito ISHIGURONobuhiro TAKADAYasuhiro YANOYosaburo OIKAWAToshirou HONDAMutsuyo GOKUDENTakashi TSUNODAMiyako TSURUMIShuji ANDOMasako ANDOHKozue SATOHiroki KAWABATA
Author information
JOURNALS FREE ACCESS

2014 Volume 65 Issue 1 Pages 13-21

Details
Abstract

Tick identification is important in control of tick-born diseases because tick-borne pathogens are often transmitted by specific tick species. In this study, we determined partial DNA sequences of the mitochondrial 16S rDNA gene (mt-rrs) for ticks including 7 genera and 39 species, and these ticks were allocated to 113 sequence types. Of the 39 species of ticks, 36 species (92.3%) were distinguishable by phylogenetic analysis of mt-rrs. This result suggests that species identification of ticks based on mt-rrs is a viable alternative to morphological identification. In order to establish a DNA database for identification of ixodid and argasid ticks in Japan, we deposited all sequence data in GenBank (from AB819156 to AB819268).

Information related to the author
© 2014 The Japan Society of Medical Entomology and Zoology
Previous article Next article
feedback
Top