Simultaneous HLA genotyping using the microtiter plate hybridization-2 method in Japanese population.

Abstract

We have established a simple and economical HLA genotyping method using the microtiter plate hybridization(MPH), which enables us to perform simultaneous DNA typing of HLA-A, -B, -C, -DRB1 and -DQB1 loci using the same PCR parameters and hybridization conditions. To discriminate HLA alleles at serological group existing in Japanese population, 24, 24, 23, 24, and 17 motif sequences were selected for HLA-A, -B, -C, -DRB1, and DQB1 genes, respectively. Using this method, 32 HLA loci can be typed within 5 h by one technologist.