HLA-A欠失によるHLA-A非発現型の発見

林 律子; 渡辺 嘉久; 石川 善英; 柏瀬 貢一; 山崎 雅子; 内野 郁世; 伊村 公良; 興津 馨; 浅井 隆善; 小松 孝良; 清水 勝則

doi:10.12667/mhc.11.11

Original paper

HLA-A null haplotype by HLA-A deletion

Ritsuko Hayashi, Yoshihisa Watanabe, Yoshihide Ishikawa, Kouichi Kashiwase, Masako Yamazaki, Ikuyo Uchino, Kimiyoshi Imura, Kaoru Okisu, Takayoshi Asai, Takayoshi Komatsu, Katsunori Shimizu

Author information

Ritsuko Hayashi
Shizuoka Red Cross Blood Center
Yoshihisa Watanabe
Japanese Red Cross Central Blood Center
Yoshihide Ishikawa
Tokyo Red Cross Blood Center
Kouichi Kashiwase
Tokyo Red Cross Blood Center
Masako Yamazaki
Shizuoka Red Cross Blood Center
Ikuyo Uchino
Shizuoka Red Cross Blood Center
Kimiyoshi Imura
Shizuoka Red Cross Blood Center
Kaoru Okisu
Shizuoka Red Cross Blood Center
Takayoshi Asai
Shizuoka Red Cross Blood Center
Takayoshi Komatsu
Shizuoka Red Cross Hamamatsu Blood Center
Katsunori Shimizu
Shimizu Children's Clinic

Keywords: HLA-A, blank, deletion, pulse field electrophoresis

JOURNAL FREE ACCESS

2004 Volume 11 Issue 1 Pages 11-15

DOI https://doi.org/10.12667/mhc.11.11

Details

Abstract

An HLA-A null haplotype was observed in a Japanese family by serological typing. The individual with HLAA31/blank were typed as HLA-A*3101 homozygote by both PCR-SSP and PCR-SBT methods. The PCR amplification of genomic DNA using an HLA-A locus-specific primer set designed with 3'- and 5'-untranslated regions produced only an approximately 3 kb fragment, which was expected from full length HLA-A. An approximately 10 kb fragment shorter than that of wild-type HLA-A was detected by pulse field electrophoresis analysis of Mlu I-digested DNA fragment. The nearest gene of HLA-A, HCGIX, was found to be 30 kb away from HLA-A on the side of the centromere. Therefore, the HLA-A null haplotype seemed to lack 10 kb including only HLA-A.

Corresponding author

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