Abstract
Various methods have been proposed for the testing of HLA antibodies in organ transplantation. These methods include cross-matching using donor lymphocytes, and panel reactive antibody (PRA) tests using synthetic beads coated with purified HLA molecules or extracted HLA antigens. However, currently, depending on the testing method, the results can be difficult to determine due to differences in detection sensitivity, differences in the classes/subclasses of immunoglobulin composing the donor-specific alloantibodies (DSA), and instances where non-HLA antibodies such as natural antibodies or autoantibodies react. In this lecture, I will introduce the testing and analysis methods that are routinely used for HLA antibody testing at our institution.
