1996 Volume 3 Issue 2 Pages 94-104
At the Eleventh International Histocompatibility Workshop (11th IHW), the HLA-Cw6 antigen was subdivided tentatively into Cw6.1 and Cw6.2 by several informative alloantisera. PCR-based DNA typing revealed a consistent correlation between the Cw* 0602 allele and the serologic Cw6.1 phenotype, and also between Cw* 1502 and Cw6.2. One (SAI0019) of our panels submitted to the 11th IHW was assigned as Cw6.2 on the basis of positive reaction to Cw6-specific sera (11th0435,11th0436) and multispecific sera (11th0432, 11th0433, 11th0434). Then we screened our typing sera to identify anti-Cw6.2 antisera using 94 panel-cells including SAI0019. One antiserum "31-603" was found to have an identical specificity to 11th0435 as assessed by the tail reaction patterns. Reassignment on 2457 cells using these two anti-Cw6.2 alloantisera, 11th0435 and 31 - 603, was carried out and 42 out of 2457 panels were defined Cw6.2. A PCR-SSP technique was employed to characterize the serologic Cw6.2 antigen at the DNA level and showed that all Cw6.2 positive cells had the Cw* 15 allele. The Cw6.2 gene frequency was 1.2% in Japanese population. Cw6.2 was in a linkage disequilibrium with Al, B51, B60, B61, DR9, and DR14, forming prominent HLA haplotypesin Japanese.
