1997 Volume 4 Issue 2 Pages 74-79
To analyze HLA-B antigens by isoelectricfocusing, multiple antigenic peptide (MAP) of the 16 amino acid sequece specific for HLA-Bα1 domain was prepared and immunized. The reactivity of the anti-HLA-Bα1 MAP serum with HLA-A, -B antigens was examined by isoelectricfocusing-immuno blot (IEF-IB). Of HLA-B antigens, the anti-serum strongly reacted with HLA-B 13, -B44, -B48. -B52, -B60, -B61, -B62 which had the identical amino acid sequence with the immunogen. But it did not react with HLA-B7, -B8, -B14, -B18, -B27, -B35, -B46, -B51, -B53, -B54, -B55, -B70, -B75 whose amino acid difference from the immunogen was in the range of 1〜6 out of the 16 amino acids. Of HLA-A antigens, it did not react with either of the HLA-A antigens examined, except that is weakly reacted with HLA-A 2, -A24. Immunization of synthetic MAP was useful to prepare anti-serum specific for HLA-B antigens for IEF-IB. Also, the anti-serum was considered to be an excellent reagent for biochemical analysis of HLA-B antigens.
