Abstract
It was previously reported in the results of the double Petri-dish assay that the growth of Aspergillus niger inoculated in a small dish was inhibited in a large Petri dish in which Serratia marcescens had been previously grown
Volatile antifungal substances emitted by Serratia marcescens were collected and identified using the gas chromatographic-mass spectrometer Volatile substance was absorbed to hydrochloric acid, and then dried by lyophilization. The residue was dissolved with a NaOH solution, and was analyzed with 28% Pennwalt 223+4% KOH column. Ammonia, aminoethane and trimethylamine were identified by mass spectra of electric ionization and of chemical ionization with methane. The gases of these amines repressed the growth of Aspergillus niger.
