Abstract
In a pure culture of Pseudomonas sp. strain DP-4, a 2, 4-dichlorophenol (DCP)-assimilator, 102cell/ml of DP-4 increased to 106cell/ml at the early stationary phase in a mineral salts medium to which no organic substrates were deliberately added. This increase was attributable to the expense of ca. 2μgC/ml of contaminated organic substrates in the medium. In the culture of DP-4 with ca. 106cell/ml of the heterotrophic microorganisms which were no DCP-degraders, the density of DP-4 did not increase in the mineral salts medium without DCP, and when DCP was added the density of DP-4 increased with the the concentration of DCP. In the DP-4 culture with the heterotrophic microorganisms, the specific growth rates (μ) of DP-4 were measured at various concentrations of DCP in the range 0.01-10μgC/ml, and μ of DP-4 was fitted to Monod's equation. The maximum specific growth rate (3.2/day) and the half saturation constant (0.11μg DCP-C/ml) were considered as kinetic parameters when DP-4 assimilated DCP as a sole source of carbon.
