Abstract
Flow cytometry with a fluorescent antibody technique was applied to detect Escherichia coli O157: H7. Samples from the Neya River in Osaka Prefecture, Japan, were analyzed by flow cytometry to determine the number of E. coli O157: H7 in river water. The numbers of E. coli O157: H7 cells in the river water samples ranged from 5×103 cells/ml to 6×104 cells/ml. These antibody-positive cells grew during cultivation in modified EC medium. These results indicate that viable E. coli O157: H7 occurs in river water.
