Isolation, Screening and Phylogenetic Identification of Endophytes from Plants in Hokkaido Japan and Java Indonesia

Abstract

To examine diversity of endophytic microbes in plants, we have devised a method for isolation. We could isolate at least one kind of microbes from all the plants examined. One hundred sixty two fungi and 166 bacteria were obtained from 57 plants in Hokkaido Japan and 86 fungi and 56 bacteria were obtained from 20 plants in Java Indonesia.
Among those isolates shown to have interesting bioactivities, 9 filamentous fungi were selected and taxonomic study was carried out on the basis of 18S rDNA base sequence analyses.
These experiments suggest that endophytes from various plants have great diversity and useful characteristics for biotechnological applications.