2020 Volume 36 Issue 2 Pages 39-48
Cellulolytic myxobacteria cultured using filter paper as the only carbon source were isolated from soils in Japan. To purify isolated strains, fruiting bodies of cellulolytic myxobacteria formed on Stanier agar medium were incubated at 60℃ for 1-7 days and/or at room temperature for 3-6 months. Purified 120 strains were cultured in P- and E-liquid media for 4-6 weeks at 28℃. Approximately 75% of the strains were found to produce metabolites with antimicrobial activity, and 63% of extracts showed growth inhibitory activity against Aspergillus niger. Based on LC-MS analysis, we estimated that 34 known natural products of the genus Sorangium were produced by 100 isolates, including spirodienal, ambruticin, disorazole, and epothilone. Also, many products whose molecular weights did not match those of known products from the genus Sorangium were detected.
