Additional polyamine analysis of the algae belonging to the phyla Glaucophyta, Rhodophyta and Chlorophyta

─Polyamine analysis of algae V─

Abstract

To prepare a cellular polyamine distribution catalogue of the three algal phyla Glaucophyta, Rhodophyta and Chlorophyta in Archaeplastida, we analyzed 2 strains (2 species) of glaucophytes, 14 strains (10 species) of red algae and 27 strains (22 species) chlorophyte green algae. The acid-extracted polyamines from the algae were analyzed by HPLC and HPGC-MS. Putrescine and spermidine were the polyamines of Cyanophora and Glaucophyra of Glaucophyta. In the unicellular acidothermophilic Rhodophyta, Cyanidium, Cyanidioschyzon and Cyanidiococcus contained putrescine, spermidine and spermie, and norspermidine, homospermidine and thermospermine were added in Galdieria. Multicellular marine Asparagopsis contained homospermidine and aminopropylhomospermidine. Caldopentamine and thermopentamine were distributed in multicellular freshwater Hildenbrandia. In the Chlorophyta, putrescine and spermidine were the major polyamines in Carolibrandtia, Picochlorum, achlorophylus Prototheca and phototrophic/heterotrophic Auxenochlorella. Norspermidine was added to endosynbiotic Protodesmus. Coccomyxa and photobiontic Asterochloris contained putrescine, norspermidine, spermidine, homospermidine and spermine. Grecesiella and bacteriovory-potent Pyramimonas and Pterosperma contained putrescine, norspermidine and spermidine. Snow alga Chloromonas contained diaminopropane, putrescine, norspermidine and spermidine. Gymnomonas contained putrescine alone.