Microvascular Reviews and Communications
Online ISSN : 1880-5906
Print ISSN : 2188-1707
ISSN-L : 2188-1707
The blood cell flow and the vascular responses in arterioles and capillaries after subarachnoid hemorrhage
Mami IshikawaMayumi KajimuraTakayuki MorikawaTomomi NakamuraYuichi TanakaEiju WatanabeMakoto Suematsu
Author information
JOURNAL FREE ACCESS

2014 Volume 7 Issue 1 Pages 35

Details
Abstract

Purpose: Immediately after subarachnoid hemorrhage (SAH), brain injury begins and determines the acute phase mortality and the long-term prognosis, but its mechanism is not well understood. When SAH at the skull base induces platelet-leukocyte-endothelial cell interactions in venules, the cerebral blood flow is kept well at the cerebral surface1). We investigated cerebral microcirculation through a mouse cranial window using two-photon laser scanning microscopy at a depth of about 100µm2,3), after SAH was induced at the skull base.
Methods: Tracheotomy was performed and femoral artery was cannulated in mice (FVB/N-Tg (GFAP GFP) 14Mes/j). Q-dot 655 nanocrystal (Q21021MP; Invitrogen) or rhodamine-6G was injected from the cannulated femoral vein, after a craniotomy at the parietal bone without cutting dura matter. SAH was induced at a prone position by using the endovascular perforation model4). Immediately and one hour after SAH, blood cell velocities were measured with a line scan method in precapillary and capillary using two-photon laser scanning microscopy.
Results: A penetrating arteriole branched into a precapillary arteriole at the depth of 85.9 +/- 21.0µm (n=7). Arterioles dilated immediately after SAH and then gradually constricted (n=5/7) and the blood flow disappeared immediately after SAH in the others (n=2/7). The blood cell velocity of the precapillary arteriole decreased from 10.7 +/- 3.0 mm/s before SAH to 0.9 +/- 0.4 mm/s after SAH. The capillary-velocities of blood cells (red blood cells, platelets and leukocytes) also decreased, and rolling and adherent leukocytes prevented blood cells from flowing in capillaries.
Conclusion: The cerebral blood flow decreases in arterioles and capillaries, when the SAH is induced.

Related papers from these authors
© 2014 by Japanese Society for Microcirculation
Previous article Next article
feedback
Top