Abstract
Decomposition of deoxynivalenol (DON) and nivalenol (NIV) during roasting of naturally contaminated barley was determined by GC-MS, and by ELISA using a monoclonal antibody which recognizes the partially acetylated derivatives of DON and NIV (3,15-diacetyl-DON and 3,4,15-triacetyl-NIV). As determined by GC-MS, DON and NIV were decomposed with the extent of reduction dependent upon temperature and processing time. By ELISA, a clear decrease in the toxin level was observed above 170 °C, whereas at 150 °C the toxin level increased after 5 and 30 min heating in spite of a slight decomposition observed by GC-MS. This phenomenon was also confirmed when the standard toxins were heated at 150 °C, indicating that heat-induced derivatives of the toxins may have a stronger cross-reactivity against the antibody.
