Abstract
The effect of industrial processing on deoxynivalenol (DON) and nivalenol (NIV) was examined in naturally contaminated wheat grains and their milling products (flour). A total 160 samples (80 samples each of wheat grains and flour) were obtained from milling companies. Among them, a pair of 20-lot samples of wheat grain and flour was divided into 4 different uses; home use, cake, noodles, and bread.
The toxins were extracted with acetonitril- water (85:15), and passed through a multi-function column for clean-up. After their trimethylsilyl derivatization, quantitative determination of the toxins was carried out by gas chromatography-mass spectrometry in the selected ion monitoring mode.
The concentration of DON and NIV in wheat grains was ND-2,450 ng/g and ND-174 ng/g, respectively. In the processed products, it was ND-1,620 ng/g for DON and ND-20 ng/g for NIV. The mean and range of reduced DON and NIV rates after processing was 74% (0-97%) and 63% (22-91%), respectively.
