JSM Mycotoxins
Online ISSN : 1881-0128
Print ISSN : 0285-1466
Research Paper
Implication of histone H10-derived 17-kDa DNase in tamoxifen-induced apoptosis in aflatoxin B1-elicited rat hepatocellular carcinoma cells
Yasushi KAWASAKINaomi ADACHIIsamu TAMURATakashi KOMENONaoyuki IIDAHirotada AKIYAMAAkinori SUGIYAMAFumio TASHIRO
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2014 Volume 64 Issue 2 Pages 117-139

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Abstract

  The 17-kDa N-terminal region (NTR) of rat histone H10 (Thr 2 - Phe107 ) was generated during TAM-induced apoptosis and acted as a DNase, for which two histidine residues at 25 and 57 were essential. The 17-kDa H10NTR was generated by the restricted cleavage of H10 with the Ca2+-dependent protease calpain-2, which was activated by TAM-induced Ca2+ influx ([Ca2+]i). The forced expression of the 17-kDa H10NTR stimulated, while its downregulation suppressed TAM-induced apoptosis. These results imply the involvement of the 17-kDa H10NTR DNase downstream of the TAM-triggered cell death signaling pathway, suggest that TAM could be a promising chemoprevention and anticancer drug for AFB1 hepatocarcinogenesis, and also provide a new insight into the biological functions of H10.

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© 2014 Japanese Society of Mycotoxicology
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