2022 Volume 72 Issue 2 Pages 85-87
Kojic acid is one of the major secondary metabolites produced by fungal species of Aspergillus section Flavi. In this study, we compared the extraction efficiency of kojic acid from agar media using the five solvents of different mixing ratio of methanol/water (100%, 75%, 50%, 25% and 0% of methanol), at two (low or high) kojic acid levels. The effect of ammonia exposure on the extraction efficiency was also examined. In all conditions tested, stable extraction was achieved by 100% methanol and 100% water, showing extraction efficiency of 75-86% with low standard deviations. The ammonia exposure did not affect the extraction efficiency when using above two solvents, indicating that 100% methanol or100% water is suitable rather than methanol/water mixture.
