Abstract
In the previous paper (Proc. Jpn. Assoc. Mycotoxicol. 19, 34 (1984)), the authors described the chemical analyses of deoxynivalenol (DON), nivalenol (NIV) and fusarenon-X in cereals. In this paper, we have revised the method in regards to shortage of analytical time. The cleanup with Florisil column (method A) is enough for quantitative determination of DON and NIV higher than 10 ppb. For determination of the toxins less than 10 ppb, further cleanup step using Sep-pak column (method B) was required. The present method B was applied to 30 samples of wheat, barley and corn. Both DON and NIV were detected in 13 samples of domestic cereals. DON alone was detected in 7 samples of corn imported from U.S.A.. As a whole, natural occurrence of DON and NIV were 80% of cereals tested.
