Abstract
Aspergillus flavus and related fungi were isolated from sugarcane field soil samples from three areas located along Nansei Shoto; Amami-Ohshima, Hateruma, and Okinawa islands in Southern Japan. Aspergillus species were isolated by dilution plate technique on Aspergillus Flavus/Parasiticus Agar (AFPA) and by plating plant debris on two different selective media, PCNB/2-aminobutane medium and Demosan/PCNB/Streptomycin medium. Among 60 Aspergillus isolates obtained from the soil samples, 17 were A. flavus, of which 12 were potential aflatoxin-producing strains, and 43 were A. parasiticus, all detecting aflatoxin production.
