Effects of ES-D3 Cells Exposed to Multi-walled Carbon Nanotubes on Cell Differentiation by Three-dimensional Culture with Tilapia Scale Collagen

Abstract

Scaffold selection is critical for regenerative medicine. Mammalian (porcine) collagen has been extensively studied as a scaffold. However, collagens derived from mammals close to humans may pose problems associated with viruses contained in scaffolds upon tissue and organ transplantation into humans. Therefore, fish scale collagen from tilapia, which inhabits tropical and subtropical regions, may be more suitable than porcine collagen as a scaffold for regenerative medicine. Tilapia collagen can be utilized in a biological safety test on biomaterials for regenerative medicine.

No report has been published on nanomaterials with tilapia collagen. Therefore, we investigated cell differentiation using tilapia collagen in a nanomaterial insoluble in body fluids. Specifically, the differentiation of ES-D3 cells exposed to multi-walled carbon nanotubes (MWCNTs) was examined in two-dimensional culture with tilapia collagen. As a result, ES-D3 cells were differentiated with tilapia collagen in the same manner as porcine collagen. MWCNTs had significant effects on the differentiation of ES-D3 cells.