Effect of Fibronectin Immobilization on Poly-L-lactic Acid Films for the Adhesion and Proliferation of Mouse Osteoblast-like Cells

Abstract

This study evaluated the influence of fibronectin (FN) immobilization on poly-L-lactic acid (PLA) films on attachment and proliferation of MC3T3-E1 osteoblast-like cells. FN immobilized PLA (FN-PLA) was prepared through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLA surface and the amino groups of FN using water soluble carbodiimide. The number of attached cells onto FN-PLA films was significantly greater than on PLA films. At 10 days, the greatest number of attached cells was observed on FN-PLA surface. After 7 days of culture, the morphological differences of attached osteoblast-like cells were observed between PLA films and FN-PLA films. The cell attached on FN-PLA films appeared more flat. On the other hand, cell attached on PLA films were more rounded. The present study revealed the effectiveness of fibronectin immobilization of PLA for cell attachment and proliferation. Immobilization of cell adhesive protein will provide a wide variety of useful application of PLA in dental clinics.