Abstract
We investigated the effects of reagent-grade and nanostructured zinc oxides on cytotoxicity and angiogenesis. Nanostructured zinc oxide was mixed with fetal carf serum and dispersed to simulate in vivo dispersion. Several reports have already been published on nanostructured zinc oxide. However, whether nanostructured zinc oxide is comparable with conventional zinc oxide is unknown. Thus, we investigated their cytotoxicity using a tissue model. In addition, we compared the effects of these zinc oxides on angiogenesis using a commercial angiogenesis model. As a result, nanostructured zinc oxide exhibited cytotoxicity almost comparable with that of reagent-grade zinc oxide. Reagent-grade zinc oxide barely caused angiogenesis at the concentrations used in the experiment. Nanostructured zinc oxide strongly inhibited angiogenesis. Dispersion was altered by serum at the lowest concentration. In the future, the effects of nanolevel dispersion control on cytotoxicity should be further investigated.
