Effects of C60 Fullerene on Cell Differentiation with EL-M3 and ES-R1-EGFP B2/EGFP Cell Lines

Abstract

The effects of nanomaterials on human reproduction and development remain unclear. Thus, their embryotoxicity should be examined to ensure the biological safety of the next generation. In the present study, the effects of C60 fullerene on cell differentiation were investigated using EL-M3 and ES-R1-EGFP B2/EGFP cell lines that require feeder cells, instead of the ES-D3 cells used for the EST method, an in vitro embryotoxicity test. As a result, the effects of C60 fullerene on cell differentiation increased in a concentration-dependent manner for both cell lines, demonstrating the absence of severe developmental toxicity. The developmental toxicity of C60 fullerene should be investigated for applications to new drugs.