Nano Biomedicine
Online ISSN : 2185-4734
Print ISSN : 1883-5198
ISSN-L : 1883-5198
Adhesion of Osteoblast-like Cells (Saos-2) on Micro-/Submicro-Patterned Apatite Scaffolds Fabricated with Apatite Cement Paste by Micro-Molding
Tsukasa AKASAKAHirofumi MIYAJINaoyuki KAGAAtsuro YOKOYAMAShigeaki ABEYasuhiro YOSHIDA
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2016 Volume 8 Issue 2 Pages 112-122


In the present study, we developed patterned apatites with grooves, pillars, and holes by micro-molding. The effects of the patterns on adhesion of the human osteoblastic cell line Saos-2 were investigated. The patterned apatites were fabricated with apatite cement paste using the micro-molding method. The number of attached cells and ratio of cell spreading were estimated by a cell adhesion assay using Saos-2. The resulting patterns of grooves, holes, and pillars at the micro-/submicro-level were easily transferred using the corresponding mold. Saos-2 cells were well orientated on the grooves and filopodia were radially elongated on pillars. The number of attached cells on the patterned apatite was higher than that on the planar apatite. Interestingly, the tendency of increasing of ratios of spreading cells was similar to that of decreasing of water contact angle on apatite pillars. These results show that the adhesion of Saos-2 cells was affected by the type and size of the apatite patterns.

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© 2016 Nano Biomedical Society
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