2000 Volume 2000 Issue 5 Pages 335-340
The development of standard material to judge cell-culture technique and cell-response is requested in cytotoxicity testing methods for biocompatibility of biomaterials. Vanadium or nickel standard solutions used in inductively coupled plasma(ICP) analysis were added separately at appropriate concentrations to Eagle’s medium and α-modified Eagle’s medium(α-medium). Vanadium or nickel particles were independently extracted in ultra-pure water and Eagle’s medium. Then, these extracted solutions were added to fresh Eagle’s and α-mediums. Murine fibroblast L929 cell and murine osteoblastic MC3T3-E1 cells were respectively cultured with these Eagle’s and α-mediums. The effect of the vanadium and nickel ions in the standard solution used in ICP analysis on cell growth were good accordance with that of the extraction using vanadium and nickel particles. Therefore, it was thought that vanadium or nickel standard solutions used in ICP analysis could be applied as a standard material in biocompatibility tests using cultured cells.
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