Rapid Liquid Chromatographic Separation of Nucleic Acid Bases and Aminophenol Isomers Using the Conventional Cation Exchange Resin

Abstract

Rapid separation ofnucleic acid. bases and aminophenol isom, ers using theconventiona strong cation exchange resin was investigated
The retention time of so 1utes was reduced by increasing the concentration of counter ion in the elueat and by add, ing ethanol to the eluent without decrasing the column g cie cy as the result of I nprovement of the selectivity The colu n ef ciency was found tgpb in higher with the ion exchange r sin of 6%degree of 6ss linking'than with that of 8%
The rapid separation of uracil, guanine, adenine and, cytgsine wa successfu1-with in Iess than.8niinutes. The oper ing condition was as fo 10w resin, AnnineX A 4(particle size, 17 2 ), 1umn, 91ass c 1umn f 150 mm 7mm d eluent 0 8 Pao1 CH3COONa, 2.5vo1%ethan.01, pH 4.8: ow rate, 2m min: column temperqture 7oeC AlsQ the separation of o, 3-and P-aminophenol was successful with in less than 15 minutes with the eluent of O.4mo1 KH2PO and20 vol%ethao1(pH 8, 84) The i: nlet column pressure was about 13kglcm2 in both cases