Synthesis of Deoxyribooligonucleotides Using Cesium Fluoride by the Phosphotriester Approach

Abstract

In the presence of excess cesium fluoride, the reaction of the fully protected 5'-0dimethoxytrityl-N-acyldeoxyribonucleoside 3'-[O-(4-chlorophenyl) O'-(5-chloro-8-quinolyl)phosphate]s with 5'-hydroxyl group of N-protected deoxyribonucleosides proceeded selectively under mild conditions to afford the corresponding dideoxyribonucleoside monophosphates in good yields. They were used to demonstrate the synthesis of tetradeoxyribonucleotides by the fragment condensation method.