1985 Volume 1985 Issue 6 Pages 1042-1049
Formyl-substituted macroreticular polymeric resins were prepared by the Friedel-Crafts reaction of poly-(styrene-co-divinylbenzene) with dichloromethyl methyl ether followed by stirring in acid solution and removal of proteins from their aqueous solution by these resins packed in a glass column was examined. By the hydrophobic interaction and the Schiff base formation between proteins and polymeric resins, proteins were strongly bound to the resin. The adsorbed proteins cannot be eluted by acid, base or urea solution. The resin bound with alkaline phosphatase showed catalytic activity and good operational stability as a immobilized enzyme. Theoretical consideration were carried out to elution profiles of protein from the polymer-packed column.
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