The Structure Analysis of Quasi-Molecular Ion of Ni edta Complex by Means of FAB MS/MS

Abstract

Ni edta Complex was measured by FAB MS (Fast Atom Bombardment Mass Spectrometry). Quasi-molecular ions, (MA+H-1-H)+, (M+H)+, and (M+A)+ were obtained by this technique. The CAD spectra of these precursor ions, (MA-FH-FH)+ miz 371, (M+H)miz 393, and (M+A)+miz 415 obtained by tandem mass spectrometry are character istic of the ion structure. Almost all the cases indicate the main eliminations of COOH(-45) and 2COOH(-90). In the case of (MA-FH+H) precursor ion, the elimination of H₂O(-18) was observed, but in the case of (M+H) precursor ion the elimination of CO (-28) as well as H₂O was also observed. But in the case of (M+A)+ precursor ion, the elimination of CO(-28)increased extremely. In the c ase of (MAd-Hd-H) precursor ion, the CAD spectra of m/z 371, miz 165 were the largest ion without containing Ni metal and it was confirmed using the Ni isotope. Moreover, nonmetal, Li₂, Na₂, and K₂ edta were also measured by FAB M S/MS. In the case of CAD of (M+H)+ ion of Na2 edta, m/z 90 (CO₂Na₂+) as the main fragment peak was turned out to be 0 combined with two Na.