Microcolumn Liquid Chromatography by Using Carbon Dioxide as the Mobile Phase

Abstract

A micro-scale chromatograph that facilitates the employment of modified carbon dioxide as the mobile phase with a single pump was assernMed. Carbon dioxide was modified in a 40 ml mixing chamber and the separation was carried out below the critical temperature of carbon dioxide. Liquid chromatographic retention behaviours of aromatic and polycyclic compounds by a liquid carbon dioxide elution were examined in this work. Relative standard deviations of the capacity factor of polycyclic aromatic hydrocarbons were 5-8%. The retention of test solutes had tendency to decrase with increasing the methanol concentration in the mobile phase when “Develosil ODS”, and silica-gel columns were used The system was applied to the resolution of enantiomers with the chiral stationary phase.