Studies on Uremic Substances Chemical Components in Uremic Peak "2a"

Abstract

For the purpose to investigate causes of uremia from view of chemical substances, studies were done to identify specific chemical components in plasma of patients with chronic renal failure. As reported previously, we have found a chromatographic peak (peak 2a) specific to uremic plasma and showed possibility of the peak to contain uremic toxins. In, this paper, the peak fraction was further fractionated to UV-absorbing subpeaks and subjected to identify their components. As a result, 10 components summarized in Table 1 were clarified and their possibilities as uremic toxins were discussed. Among them, 2-deoxy-2, 3-dehydro-Nacetylneuraminic acid [1], N²-phenylacetylglutamine [3] and quinolinic acid [4], known to be human metabolites, were likely to act as uremic toxins from their biological activity.4-[[(Carboxymethyl)amino]carbonyl]phenyl β-glucopyranosiduronic acid [5] was noteworthy as its orthoisomer was found in uremic fluid by L. Zimmerman, et al. and P. Gallice.