1987 Volume 1987 Issue 4 Pages 678-683
Chlorophyll and chlorophyll degradation products are very important compounds as indicators of bioactivity in water and/or sediments. Chlorophyll and chlorophyll degradation products were extracted from core samples collected from the Inland Sea of Japan and separated by high-performance liquid chromatography using Zorbax ODS column (250 mm x 4.6 mm I. D.) as the stationary phase and acetonitrile-acetone (45:55 v/v) as the mobile phase at a flow rate of 0.6 cm3.min-1 The effluent from the column was monitored with a spectrofluorometer by using an excitation wavelength of 410 nm and an emission wavelength of 670 nm. Chlorophyll a, pheophytin a, pheophorbide a, methylpheophorbide a, pyropheophytin a and pyropheophorbide a were identified as the predominant components in sediments. From vertical distribution of chlorophylls, it is presumed that 45 years elapsed for the accumulation of methylpheophorbide a and 85 years was required for the release of methoxycarbonyl groups such as pyropheophytin a and pyropheophorbide a.
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